Overview: Vitamin B12, also known as cobalamin, is a polycyclic compound containing trivalent cobalt. It participates in the production of bone red blood cells, prevents malignant anemia, and protects brain nerves from damage. This kit uses a microbiological method to detect the total vitamin B12 content in infant formula. After incubation at 37°C in the dark for 24-26 hours, the turbidity of the samples is measured at 620nm-630nm using a microplate reader. By comparing the sample turbidity with a standard curve, the vitamin B12 content in the sample can be quantitatively determined.

Principle: Vitamin B12 is an essential nutrient for the growth of Lactobacillus leichmannii. The culture medium and processed sample extract are added to microplate wells coated with the bacterial strain. After incubation for a certain period, the transmittance (or absorbance value) is measured. Within a specific detection range, the vitamin B12 content in the sample can be calculated based on the standard curve of vitamin B12 concentration versus transmittance (or absorbance value).