ADOS It is a raw material used for color development in a biochemical reagent kit, with CAS number 82692-96-4. It has high water solubility and is a stable aniline analogue. The pH range of color generation and oxidation reactions is wide, making it suitable for diagnostic testing and biochemical experiments. application 1. There are several advantages in colorimetric determination of hydrogen peroxide activity compared to conventional colorimetric reagents. 2. The new Trinder's reagent is stable enough to be used in both solution and experimental assembly line detection systems; 3. In the presence of hydrogen peroxide and peroxidase, a very stable purple or blue dye is formed during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH); 4. The molar absorbance of MBTH coupled dyes is 1.5-2 times higher than that of 4-AA coupled dyes; 5. The amount of substrate can be determined by colorimetric oxidation coupling reaction. test method 1. Prepare sample solution for enzymatic oxidation reaction. The pH range of the buffer should be 5.5-9.5. 2. Prepare a standard solution containing a known amount of substrate using the same buffer solution. 3. Add appropriate units of oxidase to the sample solution, and then add an equal volume of detection solution. 4. Incubate the mixture at room temperature or 37 ℃ for 30 minutes to 1 hour. 5. Prepare a standard curve and measure the substrate concentration in the sample solution. matters needing attention 1. This product needs to be placed in a dry and cool place for closed storage. It needs to be packaged in dark brown bottles in a well shaded container device; 2. ADOS is a white crystalline powder. If the color deepens, it may have grown bacteria or partially oxidized and cannot be used; 3. ADOS powder may adhere to the tube wall during dissolution, so use a low-speed centrifuge to reduce product loss; 4. The product has good solubility and can be directly dissolved in deionized water; Double distilled water or ultrapure water can be used for experiments with high requirements.