The color substrate ADOS has a good crystal shape, high purity, and a CAS of 82692-96-4. Compared with traditional color substrates, it has higher sensitivity and specificity, and is therefore widely used in diagnostic detection and biochemical experiments. Especially in gene expression analysis, protein purification, drug screening, etc., it has played a unique advantage. Below, we will focus on introducing the application of the chromogenic substrate ADOS from these aspects. The chromogenic substrate ADOS can be used to measure the expression levels of important proteins related to gene expression, such as transcription factors and RNA binding proteins. Specifically, it involves mixing the chromogenic substrate ADOS with the sample to be tested, and quantitatively measuring the expression level of the target protein by measuring the intensity of the chromogenic signal in the sample. The advantage of this method is that it is easy to operate, does not require the use of other complex instruments and equipment, and has high accuracy and repeatability. The chromogenic substrate ADOS can be used to identify and isolate proteins with specific fluorescence properties, providing basic data support for subsequent research. In the process of protein purification, the chromogenic substrate ADOS can bind to the target protein to form a chromogenic complex, and its distribution in the sample can be observed through colorimetry. This method is very fast and accurate in identifying target proteins, avoiding the influence of other factors.