When conducting electrophoresis experiments in a biological laboratory, the positive and negative electrodes undergo an electrolysis reaction. Prolonged electrophoresis experiments can cause the positive electrode to become acidic and the negative electrode to become alkaline. The use of buffering agents is very necessary at this time. It can stabilize the pH value of the solution at both poles and make the solution have a certain degree of conductivity, which is conducive to the migration of DNA molecules. Mops buffer is one of the commonly used buffering agents. Mops powder The main component in Mops buffer is Mops reagent, also known as 3-morpholine-propanesulfonic acid, with CAS number 1132-61-2. The common form is white crystalline powder. At room temperature of 25 ℃, the pH value of 1 M aqueous solution is between 2.5 and 4.0, making it an acidic solution. According to buffering theory, its buffering range is 6.5-7.9. Good water solubility, stable chemical properties, and no chemical reaction with strong alkalis or oxidants. To understand the components of mops buffer, it is necessary to understand its preparation method. The commonly used methods for preparing mops buffer in electrophoresis experiments are as follows: 1. Weigh 41.8 grams of MOPS white crystal powder reagent and dissolve it in approximately 700 milliliters of DEPC treated water; 2. Adjust the pH value of the above solution to 7.0 using 2N sodium hydroxide; 3. Add 20 milliliters of 1M sodium peroxide solution treated with DEPC and 20 milliliters of 0.5M ethylenediaminetetraacetic acid solution with a pH of 8.0 to the solution; 4. Dilute to 1 liter and filter out impurities using a filter membrane to obtain MOPS buffer solution. In summary, in addition to mops powder reagents, the components of mops buffer also include DEPC treated water, sodium hydroxide, sodium peroxyacetate, and ethylenediaminetetraacetic acid. The prepared mops solution needs to be prepared and used immediately, and any unused solution needs to be stored at room temperature in a dark place, otherwise it may turn yellow and affect the effectiveness of use. Desheng is a professional manufacturer of biological buffering agents. In addition to mops buffering agents, there are also dozens of commonly used biological buffering agents on the market, such as CAPS, TAPS, BICINE, etc. Desheng's production technology can ensure that the product appearance is white crystal powder, with good water solubility, stable process, and small batch differences. If you need it, please contact me!