Tri (hydroxymethyl) methylamino 2-hydroxypropylsulfonic acid, abbreviated as TAPSO buffer, is an important biological buffer widely used in life science research and experiments. TAPSO buffer has a series of unique chemical properties, making it play an important role in different experimental fields. TAPSO buffer is often used as gel electrophoresis buffer in protein electrophoresis. Its unique buffer capacity can stabilize the pH value of protein samples and ensure that proteins have stable charge properties in the electrophoresis process. At the same time, it can also reduce the heat generated during the electrophoresis process, ensuring the accuracy and reliability of electrophoresis. TAPSO buffer can be used as an extraction buffer in the process of DNA/RNA extraction. It can stabilize the structure of DNA/RNA molecules, protect them from enzyme degradation and external conditions, and improve the purity and extraction efficiency of DNA/RNA, providing high-quality samples for subsequent experiments. TAPSO buffer is commonly used as a reaction buffer in enzyme reactions. Its unique buffering properties can stabilize the activity and structure of the enzyme, improve the efficiency and accuracy of the enzyme reaction, and also prevent the enzyme reaction from being affected by the external environment, ensuring that the experimental results are not disturbed. TAPSO buffer, as an important biochemical buffer, has broad application value and plays an important role in protein electrophoresis, cell culture, DNA/RNA extraction, enzyme reaction, and other fields. Scientific researchers can choose appropriate TAPSO buffer according to experimental requirements to help the experiment proceed smoothly and obtain accurate and reliable experimental results.