3-cyclohexylaminopropane sulfonic acid, abbreviated as CAPS, is a common raw material for biological buffering agents and is widely used. It is mainly used to adjust the PH value of solution in biochemical detection and diagnostic reagents. In addition to being used as a buffer, CAPS can be used as a raw material in various water-based two component polyurethane and other environmentally friendly coatings to enhance the dryness, curing, and chemical resistance of the coatings; CAPS can also be used as an eluent in cation exchange chromatography, as a raw material for manufacturing welding materials, air conditioning equipment, and lithium metal. The following focuses on the application of CAPS as a buffer. Application of 3-cyclohexylaminopropanesulfonic acid (CAPS) 1. Usually, the concentration of methanol affects the transfer of high molecular weight proteins. Low concentrations are beneficial for the transfer of high molecular weight proteins, while high concentrations are beneficial, The transfer effect of low molecular weight proteins is better. After configuring CAPS as a buffer, the concentration of methanol is 0-20%, making it more suitable for high Transfer of molecular weight proteins; When the CAPS crystal powder is dissolved in deionized water and the pH value of the solution is adjusted to 11.0, CAPS can be used for fibronectin eggs White purification; 3. When the pH of the solution is 10.5, CAPS can promote the activity of alkaline phosphatase and inhibit the growth of Aeromonas; 4. CAPS is used as a diagnostic kit as a biological buffer, as a buffer for enzyme chemistry and HPLC separation of alkaline drugs; 5. As a terminal electrolyte, applied in capillary electrophoresis analysis; 6. CAPS buffer can be used to increase the retention sensitivity of sulfites during flow injection process; 7. Multiple agar media can be prepared in extreme medium for the cultivation of Streptomyces clavulniforme and the production of cephalosporins Production.